Chapter 5 (catabolic and anabolic reactions and enzymes)A catalyst is a substance that speeds up a chemical metabolic reaction. The catalyst itself is not used up as a result of its actions. Proteins that function as biological catalysts are called anabolic enzymes definition. As we saw in the nutrition and food webpage the function of proteins is determined by their amino acid composition as well as their shape. Enzymes control cellular reactions.
Chapter 5 (catabolic and anabolic reactions and enzymes) Flashcards | Quizlet
A catalyst is a substance that speeds up a chemical metabolic reaction. The catalyst itself is not used up as a result of its actions. Proteins that function as biological catalysts are called enzymes.
As we saw in the nutrition and food webpage the function of proteins is determined by their amino acid composition as well as their shape. Enzymes control cellular reactions. As you remember, reactions that break down substances and release energy are called catabolic reactions. Examples are respiration and digestion. The other types of reactions are called anabolic reactions. These reactions consume use energy. These reactions build larger, more complex, molecules from smaller ones.
Photosynthesis and muscle growth from amino acids are examples of anabolic reactions. The place where the substance fits into the enzyme is called the active site and the substance that fits into the active site is called the substrate. Enzyme action occurs when the enzyme and substrate collide. During the collision the substrate slots into the active site of the enzyme. Collisions happen because of the rapid random movement of molecules.
When the substrate joins with the enzyme the entire structure is called the enzyme-substrate complex. The enzyme is then free to join another substrate. Enzymes can be either anabolic or catabolic. The same enzyme can be used to form smaller molecules from a larger molecule or to do the opposite. An example of a catabolic enzyme is amylase. Amylase converts starch into maltose.
An example of an anabolic enzyme is DNA polymerase. This enzyme repairs rebuilds DNA. As stated previously, the substrate must fit into the enzyme at the active site. Some substrates fit nicely into the active site. This situation is called the Lock and Key Model. This is also explains why enzymes are very specific Enzyme Specificity. You need the right molecular key for a particular molecular lock. Sometimes the shape of the active site must be slightly changed.
This situation is called the Induced Fit Model. The active site alters its shape holding the substrate more tightly and straining it. An enzyme-substrate complex is formed. The substrate undergoes a chemical change and a new substance, product, is formed. The product is released from the active site. The free unaltered active site is ready to receive a fresh substrate. Enzymes are named by their substrate. The letters ase are added to the substrates name.
Enzymes lower the activation energy. This is the energy input needed to bring about the reaction. Enzymes enable the reaction to occur with less energy than would be needed if the enzyme were not present. Enzyme inhibitors are molecules that interact in some way with the enzyme to prevent it from working in the normal manner. Poisons and drugs are examples of enzyme inhibitors. Inhibitors change the shape of the enzyme and make it nonuasable to a substrate.
Inhibitors can also act as a substrate and bind to the enzyme. This prevents the enzyme from binding with its intended substrate. When this happens the enzyme is said to be denatured. This causes the collision frequency between enzyme and substrate to be low. Increasing the temperature speed up the movement of molecules and thus the collision frequency increases therefore enzyme action increases.
Human bio enzymes work best at 37 degrees Celsius. As the temperature raises the shape of the enzyme changes and the enzyme becomes denatured. Temperature above 50 degrees Celsius will denature most human enzymes. Most enzymes work best at a pH of When the pH is outside this range the enzyme will lose its shape and become denatured.
The ideal optimum pH for most enzymes is 7. As the concentration of an enzyme increases the rate of reaction also increases, provided that the substrate is in excess. At low concentration of substrate an increase in concentration will cause an increase in the rate of reaction. However, once the concentration is such that all the active sites of the enzyme are constantly in use then further increase in substrate concentration will have no effect on the rate of reaction.
Immobilized enzymes are enzymes which may be attached to each other, to insoluble materials, or enclosed in a membrane or gel. This can provide increased resistance to changes in conditions such as pH or temperature. It also allows enzymes to be held in place throughout the reaction, following which they are easily separated from the products and may be used again.
Immobilised enzymes are used in bioreactors. These procedures are used to produce many products which used to use micro-organisms. See the bacteria webpage for a discussion of bioreactors. In this method the enzyme is attached to a support. Supports can be ceramics, glass, or plastics. The enzymes are held in a gel. Sodium alginate is a common gel used. The gel allows the substrate to enter and the product to leave.
Chemically bonded to a support or to each other: See textbook page It makes for easier purification of the product as the separation of the enzymes from the products is easily accomplished. It is easy to recover and recycle the enzymes. This leads to a more economical process. The enzymes remain functional for much longer as it is a gentler process. Fructose derived from glucose: Fructose is sweeter than glucose and is used in soft drinks and other sweet products.
Enzymes are used to change penicillin into new, wider used, antibiotics. Instead of bacteria enzymes can be immobilised and used. Find free Leaving Cert flashcards here. Take a break from studying and watch Manchester United! Ireland and UK residents can enjoy four 4 free bets courtesy of Ladbrokes Sports. Learn more about this Ladbrokes promo code at RedeemBonusCode.
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